旋毛虫plancitoxin-1-like基因的序列分析与编码蛋白结构预测

doi:10.11843/j.issn.0366-6964.2018.01.025

Abstract

Abstract:

It is noteworthy that more extensive expansion of the DNase Ⅱ-like protein family (estimated 125 genes) in the genome of the parasitic nematode Trichinella spiralis (T. spiralis). However, only plancitoxin-1 possesses one predicted active site of DNase Ⅱ (HKD motif) in its C-terminus domain in 125 DNase Ⅱ-like protein family. To explore the molecular characteristics of plancitoxin-1 gene in T. spiralis, plancitoxin-1 gene was cloned by RT-PCR from total RNA of T. spiralis larvae, and analyzed with bioinformatics software. The results showed that the sequences of plancitoxin-1 cloned from T. spiralis were shorter (210 bp less) than the XM_003370715.1 sequence in GenBank, and named after plancitoxin-1-like. The gene encoded a protein of 294 amino acids. Intriguingly, there were two HKD motifs in the N-and C-termini in the cloned sequences. plancitoxin-1-like is a stable protein with a predicted molecular mass (Mr) of 33.19 kDa and theoretical pI of 9.17. By sequence alignment, plancitoxin-1-like protein revealed 35% sequence identity to previously reported DNase Ⅱ family members from other species. A total of 4 N-linked glycosylation sites, 3 O-linked glycosylation sites, 31 phosphorylation site 17 linear B cell piptope, 9 T cell binding sites, and 3 disulfide bond were predicted in plancitoxin-1-like. Analysis of secondary structure revealed that α-helix, extended strand, and random coil were 14.29% (42), 28.23% (83), and 55.10% (162), respectively. In this study, the plancitoxin-1-like gene was cloned and analyzed. The results laid the foundation for the further study on the role of development infection in T. spiralis.

CLC Number:

S852.731

LIAO Cheng-shui, WANG Xiao-li, TIAN Wen-jing, YANG Ya-dong, ZHANG Meng-ke, WANG An-qi, ZHANG Chun-jie, LIU Ming-yuan, BOIREAU Pascal, CHENG Xiang-chao. Cloning and Sequence Analysis of Plancitoxin-1-like Gene in Trichinella spiralis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 218-222.

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Cloning and Sequence Analysis of Plancitoxin-1-like Gene in Trichinella spiralis

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